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KMID : 0380020090240050483
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 5 p.483 ~ p.488
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Production of Bio-ethanol from Brown algae by Enzymic Hydrolysis
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Lee Sung-Mok
Choi In-Soon
Kim Sung-Koo
Lee Jae-Hwa
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Abstract
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The Brown-algae polysaccharide consisting of alginate and laminaran is usable as high bio-ethanol production if hydrolyzed to monomer unit. The objective of this study is to produce bio-ethanol from brown-algae using enzymatic saccharification. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM 1129 and Pachysolen tannophilus KCTC 7937 strains. The substrate used Laminaria japonica, Sargassum fulvellum and Hizikia fusiformis. We isolated a new alginate lyase and laminaran lyase producing microorganism for hydrolysis of brown-algae from southern sea of Gijang. The reducing sugar was obtained 1.90 g/L from Laminarin japonica 20 g/L that used enzyme from Bacterium antarctica. In pretreatment of the most suitable brown-algae for ethanol production, ethanol concentration of 0.93 g/L and yield of 4.65% were obtained in condition of Laminaria japonica in medium.
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KEYWORD
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brown-algae, bio-ethanol, alginate, laminaran, pretreatment
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